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Sodium arsenite impairs sperm quality via downregulating the ZMYND15 and ZMYND10

Xiangli Li1, Kaina Shen1, Dunxuan Yuan1

  • 1Lanzhou University of Public Health, Lanzhou, People's Republic of China.

Environmental Toxicology
|May 27, 2024
PubMed

Insights

Environmental pollutant arsenic exposure reduces sperm quality by decreasing ZMYND10 and ZMYND15 gene expression in male rats. This finding offers a potential marker for diagnosing infertility caused by environmental toxins.

Area of Science:

  • Reproductive Toxicology
  • Environmental Health
  • Molecular Biology

Background:

  • Zinc finger MYND-type containing 15 (ZMYND15) and ZMYND10 are crucial for spermatogenesis and sperm motility, respectively.
  • Mutations in ZMYND15 are linked to various forms of male infertility, including azoospermia and oligozoospermia.
  • The impact of environmental pollutants on ZMYND15 and ZMYND10 expression and subsequent male fertility remains largely uninvestigated.

Purpose of the Study:

  • To investigate if arsenic exposure affects male rat reproductive toxicity by altering ZMYND15 and ZMYND10 expression.
  • To examine the relationship between arsenic-induced reproductive injury and the expression of ZMYND15, ZMYND10, and their target genes.
  • To establish a potential link between environmental pollutant exposure and male infertility through specific gene regulation.

Main Methods:

  • A male rat model was established using sodium arsenite exposure to induce reproductive injury.
  • Sperm quality parameters and serum hormone levels were measured.
  • Intratesticular mRNA and protein expressions of ZMYND15, ZMYND10, and their target genes (including Tnp1) were analyzed.

Main Results:

  • Sodium arsenite exposure significantly reduced sperm quality and serum hormone levels in male rats compared to controls.
  • A significant decrease in both mRNA and protein expression of intratesticular ZMYND15 and ZMYND10 was observed following arsenic exposure.
  • While Tnp1 mRNA expression increased, the expression of other target genes regulated by ZMYND15 and ZMYND10 was reduced.

Conclusions:

  • Arsenic, an environmental pollutant, impairs male rat sperm quality by downregulating the expression of ZMYND10 and ZMYND15.
  • Reduced expression of ZMYND10, ZMYND15, and their target genes represents a potential mechanism for arsenic-induced male reproductive toxicity.
  • These findings suggest ZMYND10 and ZMYND15 may serve as diagnostic markers for male infertility linked to environmental pollutant exposure.
Keywords:
Prm1Tnp1arsenicmale fertilitysperm quality

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