Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Investigation of an ALDH1A1-Specific Inhibitor, FSI-TN42, as a Treatment for Obesity in Female Mice.

Nutrients·2026

Same author

Modeling and validation of parallel co-flows layer widths in open-capillary trigger valve systems.

bioRxiv : the preprint server for biology·2026

Same author

To the homeRNAmax: Developing an Improved Blood Self-Collection and Stabilization Platform for Remote Transcriptomic Studies.

Analytical chemistry·2026

Same author

Characterization of pre-analytical blood collection and stabilization parameters to maintain endogenous protein levels for remote blood sampling technology.

bioRxiv : the preprint server for biology·2026

Same author

Kinetics of cortisol and cortisone binding to corticosteroid binding globulin and albumin <i>in vivo</i>.

bioRxiv : the preprint server for biology·2026

Same author

From Fabrication to Flow: Impact of Print Orientation on Surface Qualities and Capillary-Driven Flow in Laser SLA-based Open Microchannels.

bioRxiv : the preprint server for biology·2026

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 25, 2025

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

Published on: September 2, 2009

Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets.

Tristan M Nicholson¹, Jodie C Tokihiro², Wan-Chen Tu²

¹Department of Urology, School of Medicine, University of Washington Seattle, Washington 98105, United States.

Biorxiv : the Preprint Server for Biology

|May 27, 2024

Summary

Vitrification offers a promising method for sperm cryopreservation, but pipetting small volumes is challenging. This study introduces an open droplet generator using microfluidics to precisely handle small sperm volumes, preserving their quality for fertility applications.

More Related Videos

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Related Experiment Videos

Last Updated: Jun 25, 2025

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

Published on: September 2, 2009

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Area of Science:

Reproductive Biology
Microfluidics
Biotechnology

Background:

Sperm cryopreservation is crucial for assisted reproduction.
Vitrification is an effective sperm preservation technique requiring small sample volumes.
Accurate pipetting of sub-microliter to microliter volumes remains a significant hurdle for vitrification adoption in fertility labs.

Purpose of the Study:

To develop and validate an open droplet generator for precise handling of small human sperm volumes.
To assess the compatibility of the microfluidic platform with human sperm samples.
To evaluate the preservation of sperm kinematics after processing with the droplet generator.

Main Methods:

Development of an open-channel microfluidic device for passive droplet generation.
Utilizing the device to generate sub-microliter to microliter volumes of purified human sperm.
Assessment of sperm kinematics (motility and viability) after processing.

Main Results:

The open droplet generator successfully produced sub-microliter to microliter volumes of human sperm samples.
Sperm kinematics were preserved after processing through the microfluidic platform.
The platform demonstrated compatibility with human sperm for cryopreservation applications.

Conclusions:

The developed open droplet generator effectively handles small human sperm volumes.
This microfluidic approach is compatible with human sperm, maintaining kinematic quality.
The platform provides a foundation for the broader implementation of sperm vitrification in fertility laboratories.