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High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

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High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

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Published on: June 11, 2013

EZ-SPOTs: A simple and robust high-throughput liquid handling platform.

Jon Albo, Shenghao Tan, Joee D Denis

Biorxiv : the Preprint Server for Biology

|May 27, 2024

Summary

This summary is machine-generated.

EZ-SPOTs offer a reusable and robust liquid handling platform for scientific experiments. This innovation simplifies fabrication and enhances throughput for applications like antibiotic susceptibility testing.

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Area of Science:

Biotechnology
Materials Science
Analytical Chemistry

Background:

Liquid handling is crucial for scientific experiments, but existing platforms like Surface Patterned Omniphobic Tiles (SPOTs) have limitations.
The original SPOTs platform required custom coatings and lacked durability, hindering widespread adoption.
There is a need for a cost-effective, robust, and easily fabricated liquid handling solution for high-throughput screening.

Approach:

Introducing EZ-SPOTs, a novel liquid handling device fabricated in under an hour using common materials: glass, a hydrophobic coating, and acrylic.
EZ-SPOTs retain the ease of use, volume flexibility, and scalability of the original SPOTs platform.
The new design incorporates a durable, abrasion-resistant coating, enabling multiple reuses of each device.

Key Points:

EZ-SPOTs fabrication is rapid and utilizes readily available components, reducing cost and complexity.
The durable coating allows for repeated use, significantly lowering consumable expenses and environmental impact.
Demonstrated application in antibiotic susceptibility testing shows high throughput and quantitative results comparable to gold standards.

Conclusions:

EZ-SPOTs provide a scalable, reusable, and cost-effective solution for advanced liquid handling in research.
The platform's robustness and ease of fabrication make it suitable for diverse experimental applications, including microbial diagnostics.
EZ-SPOTs represent a significant advancement in microfluidic device technology, enhancing experimental efficiency and data acquisition.