[Identification of Pneumocystis carinii in a patient dying of AIDS]

We compared two different techniques in the preparation of slides for the demonstration of Pneumocystis carinii in lung tissue of a patient died for AIDS. The impressions were prepared by imprinting the autopsy tissue surfaces on glass slides, while the concentrates were prepared by a partial homogenization and subsequent centrifugation of the pulmonary tissue. After staining, the number of protozoa microscopically observed resulted similar with both techniques; moreover using impression smears P. carinii were more easily visible.