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Updated: Jun 25, 2025
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Draft genome sequence data of methicillin-resistant Staphylococcus aureus, strain 4233
Madina Alexyuk1, Andrey Bogoyavlenskiy1, Yergali Moldakhanov1
1Research and Production Center for Microbiology and Virology, Bogenbay batyr. Str., 105, Almaty 050010, Kazakhstan.
Abstract:
Staphylococcus aureus is a conditionally pathogenic microorganism and one of the main causative agents of antibiotic resistant nosocomial infections. In immunocompromised people, S. aureus infection can cause folliculitis, furuncles, impetigo, osteomyelitis, septic arthritis, sepsis, endocarditis, pneumonia and meningitis. In the presented work, sequencing of a methicillin-resistant S. aureus, strain 4233, was performed on the Illumina MiSeq platform, followed by bioinformatics processing and gene annotation using SPAdes, RAST and CARD programs and databases. The submitted genome is a total of 2,790,390 bp long and contains 2759 genes, including 82 RNA genes. 33 % of the genes are functionally significant and represent 25 functional groups. Fourteen genes encoding resistance factors to 14 different types of antibacterial drugs were predicted. The information provided on the genome of S. aureus, strain 4233 will be of value in investigating the evolution and formation of antibiotic-resistant forms of S. aureus.
Insights
Sequencing the methicillin-resistant Staphylococcus aureus (MRSA) strain 4233 genome revealed 14 resistance genes. This data aids in understanding the evolution of antibiotic resistance in MRSA infections.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- Staphylococcus aureus is a opportunistic pathogen causing antibiotic-resistant nosocomial infections.
- Infections can be severe, particularly in immunocompromised individuals, leading to various systemic conditions.
Purpose of the Study:
- To sequence and analyze the genome of methicillin-resistant Staphylococcus aureus (MRSA) strain 4233.
- To identify genes associated with antibiotic resistance within this MRSA strain.
Main Methods:
- Whole-genome sequencing using the Illumina MiSeq platform.
- Bioinformatics analysis employing SPAdes, RAST, and CARD for gene annotation and resistance prediction.
Main Results:
- The MRSA strain 4233 genome is 2,790,390 bp with 2759 genes (82 RNA).
- 33% of genes are functionally significant across 25 groups.
- Fourteen genes conferring resistance to 14 different antibiotic classes were identified.
Conclusions:
- The genomic data of MRSA strain 4233 provides valuable insights.
- Facilitates research into the evolution and mechanisms of antibiotic resistance in Staphylococcus aureus.
