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Related Experiment Video

Updated: Jun 25, 2025

Author Spotlight: Analyzing Fibrosis Development in Chronic Lung Allograft Rejection Using Picrosirius Red Staining in Mouse Models
04:42

Author Spotlight: Analyzing Fibrosis Development in Chronic Lung Allograft Rejection Using Picrosirius Red Staining in Mouse Models

Published on: March 21, 2025

367

Macro-based collagen quantification and segmentation in picrosirius red-stained heart sections using light

Julian Kammerer1, Alexandra Cirnu1, Tatjana Williams1

  • 1Comprehensive Heart Failure Center, Department of Cardiovascular Genetics, University Hospital Würzburg, Würzburg, 97078, Germany.

Biology Methods & Protocols
|May 27, 2024
PubMed
Summary

A new FIJI macro, QuantSeg, offers automated collagen quantification for cardiac fibrosis analysis using Picrosirius red staining. This cost-effective tool provides robust and objective results, aiding in fibrosis research.

Keywords:
collagen quantificationheart segmentationmacro-based analysispicro-sirius red

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Area of Science:

  • Biomedical Engineering
  • Histology
  • Image Analysis

Background:

  • Picrosirius red (PSR) staining is crucial for visualizing collagen and fibrosis.
  • Current fibrosis quantification methods often lack objectivity, scalability, or require specialized equipment.

Purpose of the Study:

  • To develop a versatile FIJI macro, QuantSeg, for automated collagen quantification in cardiac tissue.
  • To implement an optional segmentation algorithm for analyzing diverse fibrosis patterns.
  • To validate the QuantSeg macro against established methods.

Main Methods:

  • Development of the QuantSeg macro for FIJI image processing software.
  • Utilizing PSR-stained cardiac tissue from wild-type and plakoglobin-knockout mice.
  • Comparison of QuantSeg results with a fluorescence microscopy-based method.
  • Application of the segmentation feature to rat hearts post-myocardial infarction.

Main Results:

  • The QuantSeg macro accurately detected fibrosis differences between knockout and control hearts.
  • QuantSeg demonstrated superior consistency over fluorescence microscopy in low collagen content samples.
  • The segmentation feature enabled automated evaluation of cardiac fibrosis patterns.

Conclusions:

  • The QuantSeg macro provides a robust, objective, and cost-effective solution for cardiac fibrosis analysis.
  • Its ease of use and novel segmentation feature offer significant potential for advancing PSR-stained tissue analysis.
  • QuantSeg facilitates the automated evaluation of diverse cardiac fibrosis patterns.