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Updated: Jun 19, 2026
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
Published on: October 6, 2011
Two-photon imaging of excitatory and inhibitory neural response to infrared neural stimulation
Peng Fu1, Yin Liu1,2, Liang Zhu1,3
1Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
Significance:
Pulsed infrared neural stimulation (INS, 1875 nm) is an emerging neurostimulation technology that delivers focal pulsed heat to activate functionally specific mesoscale networks and holds promise for clinical application. However, little is known about its effect on excitatory and inhibitory cell types in cerebral cortex.
Aim:
Estimates of summed population neuronal response time courses provide a potential basis for neural and hemodynamic signals described in other studies.
Approach:
Using two-photon calcium imaging in mouse somatosensory cortex, we have examined the effect of INS pulse train application on hSyn neurons and mDlx neurons tagged with GCaMP6s.
Results:
We find that, in anesthetized mice, each INS pulse train reliably induces robust response in hSyn neurons exhibiting positive going responses. Surprisingly, mDlx neurons exhibit negative going responses. Quantification using the index of correlation illustrates responses are reproducible, intensity-dependent, and focal. Also, a contralateral activation is observed when INS applied.
Conclusions:
In sum, the population of neurons stimulated by INS includes both hSyn and mDlx neurons; within a range of stimulation intensities, this leads to overall excitation in the stimulated population, leading to the previously observed activations at distant post-synaptic sites.
