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Granzyme B Promotes Proliferation, Migration and EMT Process in Gastric Cancer.

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Granzyme B (GZMB) is upregulated in gastric cancer and promotes tumor growth and migration. Reducing GZMB suppressed cancer cell proliferation and invasion, indicating its role in disease progression.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Granzyme B (GZMB) is implicated in various pathological processes, including cancer progression.
  • Understanding GZMB's specific role in gastric cancer (GC) is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the expression patterns and functional significance of GZMB in gastric cancer.
  • To elucidate the impact of GZMB on GC cell biology and progression.

Main Methods:

  • Quantitative real-time PCR, western blotting, and immunohistochemistry were used to assess GZMB expression in GC tissues.
  • In vitro assays (CCK-8, colony formation, EdU, migration) and in vivo xenograft models were employed to evaluate GZMB's functional effects.

Main Results:

  • GZMB mRNA and protein levels were significantly upregulated in GC tissues, correlating with advanced GC staging.
  • GZMB knockdown suppressed GC cell proliferation and migration, accompanied by decreased epithelial-mesenchymal transition (EMT) markers.
  • GZMB overexpression enhanced GC cell proliferation and migration, with increased EMT marker expression.

Conclusions:

  • Granzyme B (GZMB) promotes gastric cancer cell growth, migration, and epithelial-mesenchymal transition (EMT).
  • These findings highlight GZMB as a potential therapeutic target for gastric cancer.