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Updated: Jul 5, 2026

Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat
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Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

Published on: October 29, 2014

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Neonatal sepsis due to NDM-1 and VIM-2 co-producing Pseudomonas aeruginosa in Morocco

Dina Daaboul1,2, Marwan Osman3, Issmat I Kassem4

  • 1Team ReSIST, UMR1184, INSERM, Université Paris-Saclay, CEA, School of Medicine, OI HEALTHI, Le Kremlin-Bicêtre, France.

The Journal of Antimicrobial Chemotherapy
|May 28, 2024
PubMed
Abstract

Insights

Extremely drug-resistant Pseudomonas aeruginosa in a Moroccan NICU co-expressed NDM-1 and VIM-2 carbapenemases. This high-risk clone (ST773) showed limited treatment options, highlighting urgent public health concerns.

Area of Science:

  • Medical Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Increasing global prevalence of carbapenem-resistant Pseudomonas aeruginosa.
  • Need to understand resistance mechanisms in extremely drug-resistant (XDR) isolates.

Purpose of the Study:

  • Investigate molecular mechanisms of carbapenem resistance in an XDR P. aeruginosa isolate from a Moroccan neonatal intensive care unit (NICU).

Main Methods:

  • Identification of P. aeruginosa strain O82J1 using MALDI-TOF-MS.
  • Phenotypic antimicrobial susceptibility testing (Carba NP, NG-K5, disc diffusion, microbroth).
  • Whole-genome sequencing (Illumina and MinION) to predict resistome, sequence type, and plasmid types.

Main Results:

  • P. aeruginosa O82J1 co-expressed metallo-β-lactamases blaNDM-1 and blaVIM-2.
  • Strain belonged to high-risk clone ST773; blaVIM-2 was plasmid-borne, blaNDM-1 was chromosomally encoded.
  • 23 antimicrobial resistance genes detected, including blaPER-1 and rmtB, with susceptibility only to colistin and apramycin.

Conclusions:

  • Isolation of XDR P. aeruginosa in NICUs poses significant treatment challenges due to limited effective antibiotics.
  • Resistance to novel agents like cefiderocol and ATM/AVI is a major concern for managing Gram-negative infections.

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