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Epitope binning for multiple antibodies simultaneously using mammalian cell display and DNA sequencing.
Ning Lin1, Kotaro Miyamoto1, Takumi Ogawara1
1School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan.
Communications Biology
|May 28, 2024
Summary
Epitope Binning-seq enables simultaneous epitope analysis of multiple antibodies, simplifying antibody drug discovery. This novel platform efficiently groups antibodies by epitope similarity, accelerating the identification of promising drug candidates.
Area of Science:
- Biotechnology
- Immunology
- Drug Discovery
Background:
- Epitope binning is crucial for antibody drug discovery but conventional methods are complex.
- Current techniques require individual antibody production, limiting throughput.
Purpose of the Study:
- To establish a novel, high-throughput epitope binning platform.
- To enable simultaneous analysis of multiple antibodies for epitope similarity.
Main Methods:
- Epitope Binning-seq utilizes flow cytometry and next-generation sequencing.
- Query antibodies (qAbs) on cells are analyzed against fluorescently labeled reference antibodies (rAbs).
- Fluorescence-negative cells are sequenced to identify qAbs with similar epitopes to the rAb.
Main Results:
- The system successfully identified and binned 14 query antibodies simultaneously.
- Sensitivity and reliability were confirmed using pertuzumab and trastuzumab targeting HER2.
- The platform demonstrated effectiveness with antibodies at various abundances.
Conclusions:
- Epitope Binning-seq offers a versatile and efficient platform for antibody epitope evaluation.
- This method can significantly expedite the identification of clinically relevant antibodies.
- The platform is applicable to a wide range of antibodies and antigens.
