Influence of hydrogen bonds on state diagrams of cryoprotectant solutions
Related Experiment Video
Updated: Jun 25, 2025
Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures
Published on: June 28, 2017
1Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine. cryoo@ukr.net.
Background:
Transformation of state diagrams of cryoprotectant solutions under the influence of weak intramolecular interactions was considered.
Materials And Methods:
Phase states of aqueous glycerol and DMSO solutions within temperature range +25 to -150 degree С were studied using method of volumetric scanning tensodilatometry. Temperatures below which hydrogen bonds significantly affect crystallization-melting kinetics of such solutions were determined.
Results:
Principles for plotting of state diagram for binary solutions with weak intermolecular interaction of the components were set up. The study demonstrates that in such solutions formation of clusters based on ice microcrystals and cryoprotectant occurs. Based on the obtained results, state diagrams for glycerol and DMSO aqueous solutions were plotted. These diagrams include area of cluster phase existence and differ fundamentally from those describing eutectic crystallization.
Conclusion:
Nanostructures occurring in cryoprotectant solutions during their cooling were analyzed. Difference between these structures and classical solid phase eutectics were demonstrated. Doi.org/10.54680/fr24410110712.
Related Concept Videos
02:05Entropy and Solvation
01:04Hydrogen Bonds
02:34Comparing Intermolecular Forces: Melting Point, Boiling Point, and Miscibility
Temporary attractive forces like dispersion are present in all molecules, whether they are polar or nonpolar. They...
02:42Aqueous Solutions and Heats of Hydration
When ionic compounds dissolve in water, the ions in the solid separate and disperse uniformly throughout the solution because water molecules surround and solvate the ions, reducing the strong electrostatic forces between them. This process...
02:28Intermolecular Forces in Solutions
When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Such a solution is called an ideal solution. A mixture of ideal gases (or gases such as helium and argon,...
02:39Phase Diagrams