Synthesis and stability studies of bicyclo[6.1.0]nonyne scaffolds for automated solid-phase oligonucleotide synthesis
Kristina Karalė1,2, Martin Bollmark2, Antanas Karalius2
1Department of Biosciences and Nutrition, Karolinska Institutet Neo 141 57 Huddinge Sweden kristina.karale@ri.se.
RSC Advances
|May 30, 2024
Abstract:
Two novel bicyclo[6.1.0]nonyne (BCN) linker derivatives, which can be directly incorporated into oligonucleotide sequences during standard automated solid-phase synthesis, are reported. Stabilities of BCN-carbinol and two BCN-oligonucleotides are evaluated under acidic conditions. In addition, derivatized BCN linkers (non-acidic and acid treated) are evaluated for strain-promoted alkyne-azide cycloaddition (SPAAC).
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