Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Vaccine Production

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transition to adulthood in Duchenne Muscular Dystrophy: a systematic review with narrative synthesis on health systems, policies, and the role of health care providers.

Frontiers in public health·2026

Same author

Microcrystalline tyrosine as a novel depot-forming agent in venom immunotherapy: a pre-clinical evaluation in bee-venom allergic mice.

Frontiers in allergy·2026

Same author

NLRP1 inflammasome activation in skin equivalents reveals mechanistic insights into the roles of keratinocytes in psoriasis.

Cell death & disease·2026

Same author

Global Healthcare Study on Psoriasis (GHSP): cohort profile and first findings.

BMJ open·2026

Same author

Patient-Derived IgG Epitope Mapping of Bet v 1 Reveals Hypoallergenic Peptide Candidates for Safe and Next-Generation Allergen Immunotherapy.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same author

The IL-36 cytokine family: From barrier immunity to therapeutic target in inflammatory diseases.

Allergology international : official journal of the Japanese Society of Allergology·2026

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 30, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

A Basic Method for Formulating mRNA-Lipid Nanoparticle Vaccines in the Lab.

Natalia T Jarzebska^1,2, Julia Frei^1,3, Mark Mellett^1,3

¹Department of Dermatology, University Hospital Zurich (USZ), University of Zurich (UZH), Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)

|May 30, 2024

Summary

This summary is machine-generated.

Lipid nanoparticles (LNPs) are crucial for delivering RNA therapeutics like mRNA vaccines. This guide details a simple method for preparing effective LNP-encapsulated mRNA vaccines, enhancing therapeutic delivery.

Keywords:

Infectious diseases Lipid nanoparticles Messenger RNA Microfluidic mixing Modified nucleoside Pseudo uridine Unmodified nucleoside Vaccine

More Related Videos

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

Related Experiment Videos

Last Updated: Jun 30, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

Area of Science:

Biotechnology
Nanomedicine
Vaccinology

Background:

RNA therapeutics, including siRNA and mRNA vaccines, have shown significant clinical success.
Lipid nanoparticles (LNPs) are essential for protecting RNA from degradation and facilitating cellular delivery.
The efficacy of LNP-RNA systems depends critically on LNP synthesis methods and lipid composition.

Purpose of the Study:

To provide a detailed and simple protocol for the preparation of LNP-encapsulated mRNA vaccines.
To highlight the importance of LNP synthesis and lipid composition for therapeutic RNA delivery.

Main Methods:

Detailed step-by-step guide for LNP synthesis.
Focus on optimizing lipid composition for mRNA encapsulation.
Protocols for preparing LNP-encapsulated mRNA vaccines.

Main Results:

A straightforward and reproducible method for LNP preparation.
Demonstration of successful encapsulation of mRNA within LNPs.
Establishment of a foundation for developing effective LNP-mRNA vaccine formulations.

Conclusions:

Simple LNP preparation methods are vital for advancing RNA therapeutics.
Optimized LNP synthesis and composition are key to successful mRNA vaccine delivery.
This guide facilitates the development and production of LNP-encapsulated mRNA vaccines.