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Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners
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A deep eutectic solvent is an effective cryoprotective agent for platelets.

Lacey Johnson1, Saffron J Bryant2, Pearl Lei1

  • 1Research and Development, Australian Red Cross Lifeblood, Alexandria, NSW, Australia.

Cryobiology
|May 30, 2024
PubMed
Summary
This summary is machine-generated.

A novel deep eutectic solvent (DES) cryopreserved platelets effectively, showing similar quality to dimethyl sulfoxide (DMSO) but simplifying the process by eliminating toxic pre-removal steps.

Keywords:
CryopreservationDeep eutectic solventDimethyl sulfoxideMe2SOPlateletTransfusion

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Area of Science:

  • Biotechnology
  • Hematology
  • Cryobiology

Background:

  • Platelet cryopreservation typically uses dimethyl sulfoxide (DMSO), a toxic cryoprotective agent (CPA) requiring pre-freeze removal.
  • Less toxic alternative CPAs are sought to simplify platelet cryopreservation and reduce toxicity.

Purpose of the Study:

  • To evaluate a Proline-Glycerol (Prol-Gly 1:3) deep eutectic solvent (DES) as a platelet CPA.
  • To compare the effectiveness of DES with DMSO and no CPA for platelet cryopreservation.

Main Methods:

  • Platelets were cryopreserved at -80°C using 10% Prol-Gly 1:3 (DES), no CPA, or 5.5% DMSO (gold standard).
  • Platelet quality was assessed using flow cytometry and thromboelastography (TEG).

Main Results:

  • Post-thaw recovery was similar across all groups.
  • DES group showed higher labile platelet glycoproteins (GPIbα, GPVI) but also increased activation markers (CD62P, annexin-V).
  • Platelet function (clot strength, retraction) was better with DES than no CPA, but lower than DMSO.

Conclusions:

  • DES offers cryoprotective benefits for platelets compared to no CPA.
  • Most quality parameters for DES-preserved platelets were comparable to DMSO-preserved platelets.
  • DES's biocompatibility eliminates the need for pre-transfusion removal, simplifying the process and avoiding DMSO toxicity.