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Published on: April 17, 2014
Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins
Zhenkun Cai1, Weiyu Xie1, Zehua Bao1
1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, Zhejiang, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311215, Zhejiang, China; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
Abstract:
The application of CRISPR-Cas systems has been hindered by their requirement for long protospacer-adjacent motifs (PAMs). Recent engineering and discovery of PAM-flexible Cas proteins have substantially broadened the targetable DNA sequence space, thereby facilitating genome editing and improving derivative technologies such as gene regulation, seamless cloning, and large-scale genetic screens.

