Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Silicon Dioxide Multi-Mode Interference Spectrometers.

Micromachines·2026
Same author

Review of Recent Optofluidic Devices.

Micromachines·2026
Same author

Single molecule nanopore counting assay targeting small extracellular vesicle cargo for non-invasive monitoring of cerebral organoid development and health.

Scientific reports·2025
Same author

Universal Nanopore Sensor for Amplification-Free and Label-Free Detection of Molecular Biomarkers.

ACS sensors·2025
Same author

Air Core ARROW Waveguides Fabricated in a Membrane-Covered Trench.

Photonics·2025
Same author

Observational quality control study: insourcing multi-PCR-impact on the use of anti-infectives for patients with pleocytosis.

Neurological research and practice·2025

Related Experiment Video

Updated: Jun 25, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K

Adaptive time modulation technique for multiplexed on-chip particle detection across scales.

Vahid Ganjalizadeh1, Aaron R Hawkins2, Holger Schmidt1

  • 1School of Engineering, University of California, Santa Cruz, 1156 High Street, Santa Cruz, California, 95064, USA.

Optica
|May 31, 2024
PubMed
Summary

This study introduces a novel optofluidic biosensor technique using dual temporal excitation for ultrasensitive detection. It achieves a wide dynamic range, enabling digital and analog analysis of fluorescent targets from attomolar to nanomolar concentrations.

More Related Videos

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.3K
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.5K

Related Experiment Videos

Last Updated: Jun 25, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K
Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.3K
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.5K

Area of Science:

  • Optofluidics
  • Biosensing
  • Analytical Chemistry

Background:

  • Integrated optofluidic biosensors offer high sensitivity, detecting single particles and attomolar concentrations.
  • Achieving a wide dynamic range in biosensors often requires multiple detection methods or compromises linearity.
  • A need exists for biosensors that provide both high sensitivity and a broad dynamic range for practical applications.

Purpose of the Study:

  • To develop an analysis technique for integrated optofluidic biosensors that enables simultaneous digital and analog detection.
  • To demonstrate a method for achieving a wide dynamic range in biosensing without sacrificing linearity.
  • To enhance the versatility and performance of biosensors for point-of-use applications.

Main Methods:

  • Utilizing temporal excitation at two distinct time scales for simultaneous digital and analog fluorescent target detection.
  • Employing spectrally varying modulation frequencies and a closed-loop feedback system for rapid adjustment of excitation laser power.
  • Analyzing mixtures of fluorescent nanobeads at femtomolar and picomolar concentrations to demonstrate multiplexing capabilities.

Main Results:

  • Seamless detection of nanobeads across eight orders of magnitude, from attomolar to nanomolar concentrations.
  • Successful multiplex analysis of fluorescent nanobeads present at femtomolar and picomolar concentrations.
  • Demonstration of a biosensor technique that significantly advances performance and versatility.

Conclusions:

  • The developed optofluidic biosensor technique enables simultaneous digital and analog detection, achieving an unprecedented wide dynamic range.
  • The combination of temporal excitation, frequency modulation, and feedback control allows for sensitive and linear detection across a vast concentration spectrum.
  • This advancement is particularly significant for point-of-use biosensing applications, enhancing their practical utility and performance.