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Single cell analysis in systemic sclerosis - A systematic review.

Takemichi Fukasawa1,2, Asako Yoshizaki-Ogawa1, Atsushi Enomoto3

  • 1Department of Dermatology, Systemic sclerosis center, The University of Tokyo Graduate School of Medicine, Tokyo, Japan.

Immunological Medicine
|May 31, 2024
PubMed
Summary

Single cell analysis reveals immune cell dysfunction in systemic sclerosis (SSc). This review highlights cellular abnormalities in peripheral blood mononuclear cells (PBMCs), skin, and lungs of SSc patients, advancing disease understanding.

Keywords:
Systemic sclerosissclerodermasingle cell analysissystematic review

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Area of Science:

  • Immunology
  • Cell Biology
  • Rheumatology

Background:

  • Systemic sclerosis (SSc) is a complex autoimmune disease marked by immune abnormalities, fibrosis, and vasculopathy.
  • Recent advancements in single-cell analysis offer novel insights into cellular mechanisms underlying SSc pathogenesis.
  • Understanding cellular heterogeneity is crucial for developing targeted SSc therapies.

Approach:

  • A systematic review was conducted using four databases, adhering to PRISMA guidelines.
  • 17 studies involving 358 SSc patients were analyzed, examining various tissues including peripheral blood mononuclear cells (PBMCs), skin, and lungs.
  • Cell types investigated included immune cells (T cells, NK cells, monocytes, macrophages, dendritic cells) and non-immune cells (endothelial, fibroblasts, epithelial, smooth muscle, mesothelial cells).

Key Points:

  • Single-cell analysis reveals significant activation and dysfunction across multiple cell types in SSc.
  • Peripheral blood mononuclear cells (PBMCs) show altered immune profiles.
  • Skin and lung tissues (SSc-ILD, SSc-PAH) exhibit cellular changes linked to fibrosis and vasculopathy.

Conclusions:

  • Single-cell analysis provides a high-resolution view of cellular dysregulation in systemic sclerosis.
  • Findings indicate widespread cellular abnormalities contributing to the immune, fibrotic, and vascular features of SSc.
  • This comprehensive summary deepens the understanding of SSc at a cellular level, paving the way for future research and therapeutic strategies.