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A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Published on: October 6, 2019
[Closed-loop synthetic gene circuits for cell-based therapies]
Clélia Giraudot1, Nathalie Alazard-Dany2, Véronique Lambert3
1École normale supérieure de Lyon, Université Claude Bernard Lyon 1, Université de Lyon, Lyon, France - Université de Lyon, VetAgro Sup, Marcy-l'Étoile, France.
Abstract:
Recent advances in synthetic biology have paved the way for new cellular therapies, using cells capable of autonomously treating chronic diseases. These cells integrate a set of genes functioning in a closed-loop synthetic circuit, delivering a therapeutic effector in response to a specific pathological signal. While promising in mice, these therapies face clinical challenges related to safety and feasibility of in vivo implementation. The latest generations of synthetic circuits aim to address these issues through advanced bioengineering strategies outlined in this article.
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