Procedure for characterization of creatine kinase variants on agarose electrophoretograms

Electrophoretograms of atypical creatine kinase (EC 2.7.3.2, CK) isoenzymes may be difficult to interpret. In addition, their presence may cause discrepancies between results by immunological determinations for mass or activity and those by electrophoresis. Here we outline procedures to use in characterizing apparently atypical CK isoenzymes observed after electrophoresis, as illustrated by a CK isoenzyme variant that migrated as CK-MB.