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Updated: Jun 18, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
Published on: June 16, 2016
Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell
Julian Schmitz1, Boris Yermakov2, Alexander Grünberger2
1Multiscale Bioengineering, Faculty of Technology, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany.
Abstract:
Microfluidic single-cell cultivation (MSCC) is a powerful tool for investigating the cellular behavior of various cell types at the single-cell level. Here, we present a protocol specifically developed for the reliable and reproducible MSCC of industrially relevant Chinese hamster ovary (CHO) suspension cell lines. We summarize critical experimental steps from the initial seed train up to the final MSCC experiment, with a special focus on pre-culture management and medium preparation, device inoculation, and the establishment of a constant medium perfusion.
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