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EuHDZ25 positively affects rubber biosynthesis by targeting EuFPS1 in Eucommia leaves
Shuwen Zhang1, Yongyu Ren2, Shun Wang1
1State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
International Journal of Biological Macromolecules
|June 2, 2024
Summary
Triploid Eucommia ulmoides plants show higher trans-polyisoprene (TPI) rubber content. The EuHDZ25 gene positively regulates EuFPS1, boosting TPI biosynthesis and explaining increased rubber yields in triploids.
Area of Science:
- Plant genetics
- Biochemistry
- Materials science
Background:
- Eucommia ulmoides produces trans-polyisoprene (TPI), a valuable natural rubber.
- Understanding TPI biosynthesis is key for industrial applications.
Purpose of the Study:
- To investigate TPI content differences in diploid and triploid E. ulmoides.
- To identify genes regulating TPI biosynthesis and understand their roles.
Main Methods:
- TPI content analysis in diploid and triploid plants.
- Transcriptome sequencing and weighted gene co-expression network analysis.
- Gene overexpression, yeast one-hybrid, and dual-luciferase assays.
Main Results:
- Triploid E. ulmoides exhibited significantly higher TPI leaf content than diploids.
- EuFPS1 gene expression strongly correlated with TPI content; its overexpression increased TPI levels.
- EuHDZ25 positively regulated EuFPS1 expression, promoting TPI biosynthesis.
Conclusions:
- EuHDZ25 and EuFPS1 are crucial regulators of TPI biosynthesis in E. ulmoides.
- Upregulation of EuHDZ25 and EuFPS1 in triploids contributes to higher TPI content.
- Findings offer insights into polyploid secondary metabolite variation and rubber resource development.
