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Bioinformatics Analysis Reveals HIST1H2BH as a Novel Diagnostic Biomarker for Atrial Fibrillation-Related Cardiogenic

Wenbing Jiang1, Lelin Jiang2, Xiaoli Zhao3

  • 1Department of Cardiology, Wenzhou Integrated Traditional Chinese and Western Medicine Hospital, No.75 Jinxiu Road, Lucheng District, Wenzhou, 325000, Zhejiang Province, People's Republic of China. jiangwb919@wmu.edu.cn.

Molecular Biotechnology
|June 2, 2024
PubMed

Insights

Atrial fibrillation (AF) can lead to stroke. Researchers identified HIST1H2BH as a key biomarker for diagnosing AF-related cerebral embolism (AF-CE), offering new diagnostic potential.

Area of Science:

  • Biomarker Discovery
  • Genomics
  • Cardiovascular Research

Background:

  • Atrial fibrillation (AF) is a major risk factor for cerebral embolism.
  • Current diagnostic methods for AF-related cerebral embolism (AF-CE) require improvement.
  • Identifying novel biomarkers is crucial for early detection and management of AF-CE.

Purpose of the Study:

  • To discover novel diagnostic biomarkers for atrial fibrillation-related cerebral embolism (AF-CE).
  • To analyze gene expression data from AF and stroke patient cohorts.
  • To validate potential biomarkers using meta-analysis and statistical modeling.

Main Methods:

  • Examination of multiple Gene Expression Omnibus (GEO) datasets.
  • Meta-analysis of RNA sequencing data to identify differentially expressed genes.
  • LASSO regression and Receiver Operating Characteristic (ROC) curve analysis for biomarker selection and validation.
  • Gene Ontology (GO) and KEGG pathway enrichment analysis.
  • Protein-protein interaction (PPI) network analysis.

Main Results:

  • Differential gene expression analysis identified 16 upregulated and 4 downregulated genes in stroke patient blood samples.
  • Meta-analysis confirmed varied expression in eight genes; LASSO regression pinpointed five key genes.
  • HIST1H2BH was identified as a characteristic gene for AF-CE, with high diagnostic accuracy confirmed by ROC analysis.
  • Active pathways included integrin signaling, platelet activation, ECM interactions, and PI3K-Akt.
  • HIST1H2BH showed interaction with ITGA2B and links with NK cells and eosinophils.

Conclusions:

  • HIST1H2BH is a promising diagnostic biomarker for atrial fibrillation-related cerebral embolism (AF-CE).
  • This biomarker may influence platelet activation and thrombus formation, contributing to embolism.
  • Further research into HIST1H2BH's role can enhance AF-CE diagnosis and therapeutic strategies.

Keywords:
Atrial fibrillationBioinformaticsBiomarkerCerebral embolismHIST1H2BHImmune infiltrationMeta-analysisThrombus formation

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