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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
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Shedding Light on Intracellular Proteins using Flow Cytometry.

Sonal M Manohar1

  • 1Department of Biological Sciences, Sunandan Divatia School of Science, SVKM's NMIMS (Deemed-to-be) University, Vile Parle (West), Mumbai, 400056, India. Sonal.Manohar@nmims.edu.

Cell Biochemistry and Biophysics
|June 3, 2024
PubMed
Summary

Flow cytometry enables rapid, high-throughput analysis of intracellular protein levels in single mammalian cells. This review explores its applications and technological advancements for detecting intracellular proteins.

Keywords:
Flow cytometerFluorescenceIntracellular protein stainingPhospho-flowSingle cell analysisSubcellular Protein Localization

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Area of Science:

  • Cellular biology
  • Immunology
  • Biochemistry

Background:

  • Traditional methods like western blotting lack single-cell resolution for intracellular protein measurement.
  • Fluorescence microscopy offers single-cell data but is not suitable for high-throughput analysis.
  • Flow cytometry provides rapid, multiparameter analysis of intracellular protein expression at the single-cell level.

Purpose of the Study:

  • To review the scope and applications of flow cytometry for intracellular protein detection in mammalian cells.
  • To discuss technological advancements overcoming limitations in traditional flow cytometry for intracellular protein analysis.

Main Methods:

  • Review of existing literature on flow cytometry techniques for intracellular protein detection.
  • Discussion of customized assays developed for flow cytometric analysis.
  • Exploration of technological advancements in flow cytometry.

Main Results:

  • Flow cytometry is a powerful tool for high-throughput, single-cell analysis of intracellular proteins.
  • Numerous customized assays facilitate the detection of specific intracellular proteins.
  • Technological progress continues to enhance the capabilities of flow cytometry for this application.

Conclusions:

  • Flow cytometry is a versatile and essential technique for studying intracellular protein abundance in mammalian cells.
  • Its ability to provide rapid, high-throughput, single-cell data makes it invaluable for various research areas.
  • Ongoing technological developments promise further improvements in intracellular protein analysis using flow cytometry.