Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning
Taiki Kuzuhara1,2, Kota Monden1,2, Takushi Hachiya1,2,3
1Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, Japan.
Bioscience, Biotechnology, and Biochemistry
|June 4, 2024
Abstract:
Gateway cloning is a useful technology for the simple and reliable preparation of various construct in many organisms. However, there is a problem regarding the negative control construct in the Gateway cloning system. In this study, we developed the pENTR-NeCo-lacZα vector system to create an empty vector that can be used as a negative control construct in Gateway cloning.


