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Related Experiment Videos

Protease inhibitors in human milk.

T Lindberg

    Pediatric Research
    |September 1, 1979
    PubMed
    Summary

    Human milk contains protease inhibitors, primarily alpha 1-antitrypsin and antichymotrypsin, which protect against certain enzymes. These inhibitor levels are highest at birth and decrease significantly within the first week postpartum.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Human Lactation Studies

    Background:

    • Human milk contains various bioactive components crucial for infant development and protection.
    • Protease inhibitors play a role in regulating enzymatic activity, potentially offering protective benefits.

    Purpose of the Study:

    • To identify and quantify protease inhibitors in human milk.
    • To investigate the presence and activity of trypsin, chymotrypsin, elastase, and pepsin inhibitors in early postpartum milk.

    Main Methods:

    • Agarose gel electrophoresis was used to localize protease inhibitors.
    • Crossed immunoelectrophoresis and electroimmunoassay identified specific inhibitors like alpha 1-antitrypsin and antichymotrypsin.
    • Enzymatic methods assessed the inhibitory capacity against trypsin.

    Main Results:

    • Protease inhibitors targeting trypsin, chymotrypsin, and elastase were detected in human milk from birth to 4 months postpartum.
    • No pepsin inhibitor activity was found.
    • Alpha 1-antitrypsin and antichymotrypsin were identified as key inhibitors, with concentrations significantly higher in colostrum compared to mature milk.
    • Human milk demonstrated substantial trypsin inhibitory capacity, particularly in the first few days postpartum.

    Conclusions:

    • Human milk possesses significant protease inhibitory activity, mainly attributed to alpha 1-antitrypsin and antichymotrypsin.
    • The high levels of these inhibitors in early milk suggest a protective role for the newborn infant.
    • Further research could explore the clinical implications of these findings for infant health.

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