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Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

171
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
171
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

188
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
188
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

685
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
685
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.0K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

564
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
564
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

658
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
658

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Diverse Applications of Two-Dimensional Correlation Spectroscopy (2D-COS).

Yeonju Park1, Isao Noda2, Young Mee Jung1

  • 1Department of Chemistry, Institute for Molecular Science and Fusion Technology, and Kangwon Radiation Convergence Research Support Center, Kangwon National University, Chuncheon, Korea.

Applied Spectroscopy
|June 5, 2024
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Summary
This summary is machine-generated.

This review highlights the expanding uses of two-dimensional correlation spectroscopy (2D-COS) in diverse scientific fields. Infrared and fluorescence spectroscopy are popular probes, with environmental science applications growing rapidly.

Keywords:
2D-COSTwo-dimensional correlation spectroscopyreviewsurvey

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Area of Science:

  • Spectroscopy
  • Analytical Chemistry
  • Environmental Science

Background:

  • Two-dimensional correlation spectroscopy (2D-COS) is a powerful technique for analyzing complex systems.
  • This review focuses on recent advancements and applications of 2D-COS.

Purpose of the Study:

  • To provide a comprehensive survey of 2D-COS applications over the last two years.
  • To identify trends in probes, perturbations, and scientific systems studied.

Main Methods:

  • Literature review of studies utilizing 2D-COS.
  • Analysis of popular spectroscopic probes and perturbation types.

Main Results:

  • Infrared spectroscopy remains the most popular probe for 2D-COS.
  • Fluorescence spectroscopy shows significant application in heavy metal binding and environmental studies.
  • Concentration, composition, pH, and temperature are key perturbations, with environmental science being a major focus area.

Conclusions:

  • 2D-COS is continuously evolving with novel developments.
  • The technique demonstrates successful and diverse applications across various scientific disciplines.