Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Crystallography02:18

X-ray Crystallography

23.9K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
23.9K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.8K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stem cell preservation with novel cryoprotectants.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same author

Insights into the molecular association of aqueous deep eutectic solvents using cell permeability.

Physical chemistry chemical physics : PCCP·2026
Same author

Engineered nanoparticles from egg and milk proteins: A review of aggregation, gelation, colloidal behaviors and delivery systems.

International journal of biological macromolecules·2025
Same author

Heavy-element damage seeding in proteins under XFEL illumination.

Journal of synchrotron radiation·2025
Same author

Crystal structures of protic ionic liquids.

The Journal of chemical physics·2025
Same author

Alginate-ionic liquid injectable hydrogels supporting protein crystallization.

International journal of biological macromolecules·2025
Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026
Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects
09:16

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects

Published on: June 8, 2016

16.2K

Crystal structure via fluctuation scattering.

Patrick Adams1, Tamar L Greaves1, Andrew V Martin1

  • 1RMIT University, Melbourne, VIC 3000, Australia.

Iucrj
|June 6, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a new iterative algorithm to determine crystal structures from fluctuation X-ray scattering data. This method enables structure determination from nanocrystals and crystal ensembles, potentially lowering sample size requirements for crystallography.

Keywords:
Bragg peak intensityX-ray cross-correlation analysiscorrelated fluctuationsfluctuation X-ray scatteringiterative projection algorithmsmolecular crystalsserial crystallographystructure-factor intensities

More Related Videos

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.7K
Author Spotlight: Advancing Protein Structure Analysis for Drug Development
07:08

Author Spotlight: Advancing Protein Structure Analysis for Drug Development

Published on: March 8, 2024

3.5K

Related Experiment Videos

Last Updated: Jun 24, 2025

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects
09:16

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects

Published on: June 8, 2016

16.2K
Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.7K
Author Spotlight: Advancing Protein Structure Analysis for Drug Development
07:08

Author Spotlight: Advancing Protein Structure Analysis for Drug Development

Published on: March 8, 2024

3.5K

Area of Science:

  • Crystallography
  • X-ray scattering
  • Structural biology

Background:

  • Crystallography typically requires large single crystals (micrometre-sized) for atomic structure determination.
  • Serial data collection methods offer new possibilities for nanocrystals and crystal ensembles.
  • Fluctuation X-ray scattering (FXS) is used for single-particle imaging but not yet for crystal structure determination.

Purpose of the Study:

  • To develop an iterative algorithm for recovering crystal structure-factor intensities from FXS correlations.
  • To demonstrate the algorithm's capability in determining the structure of small-molecule and protein crystals.

Main Methods:

  • An iterative algorithm was developed to process FXS correlation data.
  • Simulated FXS correlation data from small-molecule and protein crystals were used for testing.

Main Results:

  • The algorithm successfully recovered crystal structure-factor intensities from simulated FXS data.
  • The crystal structures of three small-molecule crystals and one protein crystal were accurately determined.

Conclusions:

  • The presented algorithm enables crystal structure determination using FXS data.
  • This method can be applied to serial crystallography experiments and may reduce sample size requirements.