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Updated: Jun 24, 2025
06:55
Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
Published on: September 26, 2016
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[n]Cycloparaphenylenes as Compatible Fluorophores for Melt Electrowriting
Patrick C Hall1, Harrison W Reid2, Ievgenii Liashenko1
1Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA.
Small (Weinheim an Der Bergstrasse, Germany)
|June 6, 2024
Summary
[n]cycloparaphenylenes ([n]CPPs) are effective fluorescent probes for melt electrowriting (MEW) bioimaging. These probes withstand MEW
Area of Science:
- Materials Science
- Bioimaging
- Polymer Chemistry
Background:
- Fluorescent probes are crucial for bioimaging biomaterial integrity.
- Melt electrowriting (MEW) requires high temperatures, challenging fluorophore integration.
- Need for robust fluorophores compatible with MEW processing.
Purpose of the Study:
- To evaluate [n]cycloparaphenylenes ([n]CPPs) as fluorescent probes for MEW.
- To assess the thermal stability and biocompatibility of [n]CPP-PCL blends.
- To demonstrate the utility of [n]CPP-PCL blends in multi-color biofabrication.
Main Methods:
- Incorporation of [n]CPPs into poly(ε-caprolactone) (PCL) via melt electrowriting.
- Thermal stability testing of [n]CPP-PCL blends at 80°C for one week.
- Cytotoxicity assays and counterstaining with DAPI, rhodamine, and fluorescein phalloidin.
- Sequential deposition of different colored [n]CPP-PCL MEW fibers.
Main Results:
- Three [n]CPP-PCL blends with distinct emission wavelengths (466, 494, 533 nm) were prepared at 0.01 wt% concentration.
- [n]CPPs exhibited excellent dispersion in PCL and maintained fluorescence after prolonged heating.
- The blends showed no cytotoxicity and were compatible with common bio-stains.
- Successful fabrication of multi-color scaffolds and patterned structures using [n]CPP-PCL fibers.
Conclusions:
- [n]Cycloparaphenylenes are highly effective and thermally stable fluorophores for melt electrowriting.
- These [n]CPP-PCL blends offer versatile, customizable imaging options for MEW-based biofabrication.
- This work expands imaging capabilities in MEW technologies.
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