Kiwi-Inspired Rational Nanoarchitecture with Intensified and Discrete Magneto-Fluorescent Functionalities for
Daquan Li1, Lijiao Ao2, Rong Hu1
1College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China.
Small (Weinheim an Der Bergstrasse, Germany)
|June 7, 2024
Summary
This study introduces a novel magneto-fluorescent silica nanohybrid for ultrasensitive fluorescent lateral flow immunoassays (FLFIA). The new platform achieves highly sensitive detection of biomarkers, improving upon existing FLFIA methods.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Analytical Chemistry
Background:
- Fluorescent lateral flow immunoassays (FLFIA) are established for rapid quantitative analysis.
- Detecting biomarkers at pg mL-1 levels with FLFIA remains challenging.
Purpose of the Study:
- To develop an ultrasensitive FLFIA platform for low-level biomarker detection.
- To utilize a novel kiwi-type magneto-fluorescent silica nanohybrid (MFS) for enhanced signal amplification and target enrichment.
Main Methods:
- Fabrication of a spatially-layered MFS nanohybrid with Fe3O4 core, dendritic mesoporous silica, quantum dots, and silica matrix.
- Implementation of liquid-phase capturing, fluorescence enhancement, magnetic enrichment, and magnetic separation for signal amplification and noise reduction.
- Application of the MFS-based FLFIA for cardiac troponin I detection.
Main Results:
- The MFS nanohybrid exhibited enhanced fluorescence, rapid magnetic response, and good dispersibility.
- The MFS-based FLFIA achieved a limit of detection of 8.4 pg mL-1 for cardiac troponin I.
- This represents a significant improvement over existing fluorescent and colorimetric lateral flow immunoassays.
Conclusions:
- The MFS nanohybrid offers a promising strategy for magneto-fluorescent synergetic signal amplification in LFIA.
- This approach enables highly sensitive, rapid, and on-site diagnosis of biomarkers.
- The developed platform has significant potential for clinical diagnostics.
