Hyperspectral imaging for quantifying Magnaporthe oryzae sporulation on rice genotypes

Precise evaluation of fungal conidia production may facilitate studies on resistance mechanisms and plant breeding for disease resistance. In this study, hyperspectral imaging (HSI) was used to quantify the sporulation of Magnaporthe oryzae on the leaves of rice cultivars grown under controlled conditions. Three rice genotypes (CO 39, Nipponbare, IR64) differing in susceptibility to blast were inoculated with M. oryzae isolates Guy 11 and Li1497. Spectral information (450-850 nm, 140 wavebands) of typical leaf blast symptoms was recorded before and after induction of sporulation of the pathogen.

M. oryzae produced more conidia on the highly susceptible genotype than on the moderately susceptible genotype, whereas the resistant genotype resulted in no sporulation. Changes in reflectance spectra recorded before and after induction of sporulation were significantly higher in genotype CO 39 than in Nipponbare. The spectral angle mapper algorithm for supervised classification allowed for the classification of blast symptom subareas and the quantification of lesion areas with M. oryzae sporulation. The correlation between the area under the difference spectrum (viz. spectral difference without and with sporulation) and the number of conidia per lesion and the number of conidia per lesion area was positive and count-based differences in rice - M. oryzae interaction could be reproduced in the spectral data.

HSI provided a precise and objective method of assessing M. oryzae conidia production on infected rice plants, revealing differences that could not be detected visually.