Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

706
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
706
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

980
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
980

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges and future directions of AIRR-seq-based diagnostics.

Immunoinformatics (Amsterdam, Netherlands)·2026
Same author

Observational epidemiological studies can mitigate genetic confounding with a genetic relatedness matrix.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Phenotypic complexity determines the predictability of molecular convergence.

bioRxiv : the preprint server for biology·2026
Same author

Discovery of additional ancient genome duplications in yeasts.

Current biology : CB·2026
Same author

Characterizing and mitigating confounding by unreplicated evolutionary events in phylogenetic regression.

bioRxiv : the preprint server for biology·2026
Same author

Biophysical constraints on mRNA decay rates shape macroevolutionary divergence in steady-state abundances.

bioRxiv : the preprint server for biology·2025
Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026
Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026
Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026
Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
15:07

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

Published on: December 28, 2015

26.7K

Evaluating methods for B-cell clonal family assignment.

Katalin Voss1, Katrina M Kaur2, Rituparna Banerjee3

  • 1Department of Quantitative and Computational Biology, University of Southern California, USA.

Biorxiv : the Preprint Server for Biology
|June 10, 2024
PubMed
Summary
This summary is machine-generated.

Comparing B-cell receptor repertoire analysis methods, SCOPer-H excels but requires germline gene references. The mPTP method shows promise for antibody evolution studies in species lacking such references.

More Related Videos

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
00:09

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

10.7K
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
13:14

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

10.4K

Related Experiment Videos

Last Updated: Jun 24, 2025

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
15:07

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

Published on: December 28, 2015

26.7K
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
00:09

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

10.7K
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
13:14

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

10.4K

Area of Science:

  • Immunology and Bioinformatics
  • Adaptive immune response
  • B-cell receptor repertoire analysis

Background:

  • B-cell receptors are crucial for adaptive immunity, generated by VDJ recombination and refined by somatic hypermutations.
  • Accurate clonal identification is essential for understanding B-cell expansion dynamics and somatic hypermutation patterns.
  • Existing methods for B-cell clone identification lack comprehensive comparative analysis.

Purpose of the Study:

  • To conduct an extensive comparison of current B-cell repertoire analysis methods.
  • To evaluate the performance of the mPTP (motile PTP) phylogenetics method for B-cell clonal assignment.
  • To assess errors in clonal assignment and ancestral state reconstruction across different methods.

Main Methods:

  • Extensive simulations of B-cell repertoires under diverse conditions.
  • Comparative analysis of clonal assignment accuracy using SCOPer-H and mPTP.
  • Evaluation of downstream ancestral state reconstruction errors.

Main Results:

  • SCOPer-H demonstrated superior performance across various parameters for clonal assignment.
  • The mPTP method exhibited lower error rates compared to tailor-made immunogenetic methods.
  • SCOPer-H's reliance on germline gene assembly limits its applicability in non-model organisms.

Conclusions:

  • SCOPer-H is a highly effective method for B-cell repertoire analysis when germline references are available.
  • mPTP offers a viable alternative with competitive accuracy, particularly for antibody evolution studies in species lacking reference genomes.
  • Researchers studying antibody evolution in non-model organisms should consider mPTP for reliable clonal analysis.