Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 24, 2025

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

47.9K

Producing Human Livers From Human Stem Cells Via Blastocyst Complementation.

Boyukkhanim Ahmadzada1, Philipp Felgendreff1, Anna M Minshew1

  • 1Research Trainee in the Division of Surgery Research (Ahmadzada; limited tenure), Artificial Liver and Liver Transplantation Laboratory (Minshew, Amiot, and Nyberg), and Division of Surgery Research (Nyberg), Mayo Clinic, Rochester, Minnesota, USA; Research Fellow in the Division of Surgery Research (Felgendreff), Mayo Clinic School of Graduate Medical Education, Mayo Clinic College of Medicine and Science, Rochester, Minnesota, USA. Dr Felgendreff is also affiliated with the Department of General, Visceral and Transplant Surgery, Hannover Medical School, Hannover, Germany.

Current Opinion in Biomedical Engineering
|June 10, 2024
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Refining oncological resectability for intrahepatic cholangiocarcinoma: Radiological assessment of mediastinal lymph node enlargement does not reflect clinical prognosis after curative resection.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2026
Same author

Intraoperative and ex vivo measurement of liver hardness and its correlation to radiodensity.

Current problems in surgery·2026
Same author

Morphological characterization of a novel model of steatohepatitis in the <i>FAH</i><sup><i>-/-</i></sup> pig.

Veterinary pathology·2026
Same author

Living Paired Exchange Donation: Unlocking New Horizons in Kidney Transplantation.

Mayo Clinic proceedings·2025
Same author

Machine Perfusion in Liver Transplantation: Highlights From the International Liver Transplantation Society Congress 2025.

Artificial organs·2025
Same author

Two-Stage Hepatectomy for Cholangiocarcinoma Under Total Vascular Exclusion with Veno-venous ECMO Bypass and Controlled Closed-loop In Situ Hypothermic Oxygenated Perfusion of the Liver: The Hannover CLIP-Concept.

Annals of surgical oncology·2025
Same journal

Beyond The Nucleus: Translating Engineered Protein Localization To Chromatin Modifying Enzymes.

Current opinion in biomedical engineering·2026
Same journal

Engineering Lymphatic Vessels and Lymphoid Microenvironments In Vitro to Investigate Immune Cell Trafficking.

Current opinion in biomedical engineering·2026
Same journal

Next generation technologies for CRISPR-based epigenome and transcriptional modulation.

Current opinion in biomedical engineering·2026
Same journal

Bioengineering gradients for controlled embryo and organ modeling.

Current opinion in biomedical engineering·2026
Same journal

Hyaluronic acid-based models of the brain microenvironment: Challenges and advances.

Current opinion in biomedical engineering·2026
Same journal

Interplay between extracellular matrix mechanics and cell function in mechanobiology.

Current opinion in biomedical engineering·2026
See all related articles
This summary is machine-generated.

Blastocyst complementation, a method using interspecies chimeric embryos, shows promise for generating organs for transplantation. This technique faces challenges including ethical considerations and optimizing donor cell contribution for effective organ development.

Area of Science:

  • Regenerative Medicine
  • Developmental Biology
  • Bioengineering

Background:

  • Organ transplantation is limited by donor organ scarcity.
  • Blastocyst complementation offers a novel approach to organ generation.
  • This technique involves creating interspecies chimeras to produce specific organs.

Purpose of the Study:

  • To review recent advancements in blastocyst complementation for organ production.
  • To highlight progress and challenges in creating organs for transplant using this method.

Main Methods:

  • Utilizing interspecies blastocyst complementation to generate chimeric embryos.
  • Regulating donor cell contribution within the chimeric system.
  • Addressing challenges such as cell apoptosis and ethical concerns.
Keywords:
apoptosisblastocyst complementationchimeric embryosethical concernsgene knockoutliverpigtransplant

More Related Videos

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
11:44

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Published on: August 29, 2016

10.9K
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
06:57

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Published on: July 20, 2019

8.9K

Related Experiment Videos

Last Updated: Jun 24, 2025

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

47.9K
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
11:44

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Published on: August 29, 2016

10.9K
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
06:57

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Published on: July 20, 2019

8.9K

Main Results:

  • Demonstrated potential for regulated donor cell contribution in chimeric systems.
  • Identified ethical considerations, particularly regarding brain tissue.
  • Noted limitations due to low donor cell ratios and apoptosis in some chimera productions.

Conclusions:

  • Blastocyst complementation is a rapidly advancing field with significant potential for addressing organ shortages.
  • Further research is needed to overcome technical and ethical hurdles for clinical application.