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Highly-selective optical filter for NADH fluorescence detection in multiphoton microscopy.

R B Freitas1, M J L F Rodrigues2, S Pimenta1,3

  • 1CMEMS-UMinho, University of Minho, 4800-058, Guimarães, Portugal.

Biomedical Optics Express
|June 10, 2024
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Summary

A new optical filter precisely detects nicotinamide adenine dinucleotide (NADH) fluorescence using two-photon excitation fluorescence (TPEF) for improved colorectal cancer (CRC) detection. This technology aids in developing non-invasive optical biopsies for earlier diagnosis.

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Area of Science:

  • Biomedical Optics
  • Materials Science
  • Cancer Diagnostics

Background:

  • Colorectal cancer (CRC) poses a significant global health challenge.
  • Early detection of CRC is crucial for effective treatment and improved patient outcomes.
  • Current diagnostic methods can be invasive and lack real-time molecular information.

Purpose of the Study:

  • To develop and characterize a novel optical filter for precise detection of nicotinamide adenine dinucleotide (NADH) fluorescence.
  • To enable the use of two-photon excitation fluorescence (TPEF) for enhanced colorectal tissue analysis.
  • To advance the integration of multiphoton microscopy into colonoscopy for non-invasive optical biopsy.

Main Methods:

  • Fabrication of an optical filter using silicon dioxide and titanium dioxide thin films in a Fabry-Perot structure.
  • Characterization of the filter's optical properties, including peak transmittance and full-width at half maximum.
  • Two-photon excitation fluorescence (TPEF) measurements using a tailored setup with NADH liquid phantoms.

Main Results:

  • The developed filter exhibited a peak transmittance of approximately 95% at 483 nm.
  • The filter demonstrated a narrow bandwidth with a full-width at half maximum of 12 nm.
  • The filter effectively isolated NADH fluorescence, minimizing interference from other potential fluorophores in biological samples.

Conclusions:

  • The developed optical filter is highly effective for selective NADH fluorescence detection via TPEF.
  • This technology represents a significant advancement towards integrating optical biopsy into colonoscopy for colorectal tissue analysis.
  • Further experimental setup refinements are necessary to improve tissue differentiation and enhance CRC diagnostic capabilities.