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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
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Mycobacteriophage maravista: a cluster F1 phage discovered on Cape Cod, Massachusetts
Charles Pelagalli1, Debbie-Jacobs Sera2, Joseph A DeGiorgis3,4
1Department of Chemistry and Biochemistry, Providence College, Providence, Rhode Island, USA.
Microbiology Resource Announcements
|June 11, 2024
Abstract:
Mycobacterium virus Maravista, a member of the family Gracegardnervirianae and species Cheoctovirus, is an F1 cluster phage that infects Mycobacterium smegmatis mc²155. The Maravista genome has 61.3% GC content, is 60,140 bp in length, and encodes 104 putative genes. Maravista encodes two putative glycosyltransferases, suggesting glycosylation of its capsid protein.
Insights
Mycobacterium virus Maravista is a novel phage infecting Mycobacterium smegmatis. Its genome analysis reveals potential glycosylation of its capsid protein, offering insights into phage-host interactions.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Mycobacterium smegmatis is a key model organism for studying mycobacteria.
- Bacteriophages are viruses that infect bacteria and play roles in microbial evolution and biotechnology.
- Understanding phage diversity and function is crucial for various applications.
Purpose of the Study:
- To characterize the novel F1 cluster phage, Mycobacterium virus Maravista.
- To analyze the genomic features of Maravista and identify potential functional genes.
- To investigate potential mechanisms of capsid protein modification.
Main Methods:
- Genomic DNA extraction and sequencing of Mycobacterium virus Maravista.
- Bioinformatic analysis of the Maravista genome, including gene prediction and functional annotation.
- Comparative genomic analysis with related phages.
Main Results:
- Maravista is an F1 cluster phage infecting Mycobacterium smegmatis mc²155.
- The Maravista genome is 60,140 bp with 61.3% GC content, encoding 104 putative genes.
- Two putative glycosyltransferases were identified, suggesting capsid protein glycosylation.
Conclusions:
- Mycobacterium virus Maravista represents a new addition to the F1 cluster phage family.
- The genomic data provides a foundation for further functional studies of Maravista.
- The predicted glycosylation mechanism may influence phage-host interactions and virion stability.
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