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Genome sequence of Mycobacterium abscessus phage P3MA
Antonio Broncano-Lavado1,2, Françoise Roquet-Banères3, Laurent Kremer3,4
1Department of Clinical Microbiology, Instituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS FJD), Madrid, Spain.
Microbiology Resource Announcements
|May 22, 2025
Summary
A new bacteriophage, P3MA, was discovered in Spain infecting Mycobacterium abscessus. Its genome analysis reveals close relation to other phages in Cluster HB.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Mycobacteriophage P3MA is a novel bacteriophage.
- It was isolated using Mycobacterium abscessus 330 as the host strain.
- The isolation occurred in the Manzanares River, Madrid, Spain.
Purpose of the Study:
- To characterize the newly isolated Mycobacteriophage P3MA.
- To analyze the genomic features of P3MA.
- To determine the phylogenetic relationship of P3MA to other known phages.
Main Methods:
- Isolation of Mycobacteriophage P3MA from environmental samples.
- Genomic DNA extraction and sequencing of P3MA.
- Bioinformatic analysis of the P3MA genome, including gene prediction and comparative genomics.
Main Results:
- P3MA possesses a genome of 41,151 base pairs.
- The genome contains 63 predicted protein-coding genes.
- P3MA is phylogenetically closely related to prophages within Cluster HB of Mycobacterium abscessus.
Conclusions:
- Mycobacteriophage P3MA represents a new addition to the known mycobacteriophage repertoire.
- The genomic data places P3MA within the Cluster HB group.
- Further research can explore P3MA's potential applications in phage therapy or diagnostics.
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