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Related Experiment Videos

Thyroid-function assessment by use of enzyme immunoassays.

M T Proença, L S de Moura

    Clinical Chemistry
    |May 1, 1985
    PubMed
    Summary

    This study establishes normal reference intervals for thyroid hormones (thyroxin, triiodothyronine) and thyrotropin in children using enzyme immunoassay. Results showed minimal differences between plasma and serum for thyroxin and triiodothyronine, but significant differences for thyrotropin.

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    Preliminary data on an immunoenzymatic assay of free thyroxin.

    Clinical chemistry·1986

    Area of Science:

    • Pediatric Endocrinology
    • Clinical Chemistry
    • Immunoassay Development

    Background:

    • Accurate thyroid hormone reference intervals are crucial for diagnosing pediatric thyroid disorders.
    • Enzyme immunoassays offer a viable alternative to radioimmunoassays for thyroid function testing.

    Purpose of the Study:

    • To determine normal reference intervals for thyroxin, triiodothyronine, and thyrotropin in pediatric populations.
    • To compare enzyme immunoassay results with established radioimmunoassay data.
    • To assess differences in thyroid-stimulating hormone (thyrotropin) levels between plasma and serum samples.

    Main Methods:

    • Enzyme immunoassay (EIA) was employed to measure thyroxin, triiodothyronine, and thyrotropin levels.
    • Samples were collected from a cohort of children aged newborn to 15 years.
    • Comparison of EIA results with radioimmunoassay (RIA) data was performed.

    Main Results:

    • Enzyme immunoassay yielded results comparable to radioimmunoassay for thyroid hormone determination.
    • No significant differences were observed in thyroxin and triiodothyronine concentrations between plasma and serum.
    • Thyrotropin concentrations showed statistically significant differences between plasma and serum (p < 0.05).

    Conclusions:

    • Enzyme immunoassay is a reliable method for establishing pediatric thyroid hormone reference intervals.
    • Plasma and serum are interchangeable for thyroxin and triiodothyronine measurements in children.
    • Plasma and serum are not interchangeable for thyrotropin measurements in pediatric populations due to significant differences.

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