Requirement of zinc in human subjects

Essentiality of zinc in nutrition of higher animals was established in 1934. Dietary zinc deficiency in humans was recognized in 1961. Dietary requirements for zinc have been estimated factorially and by balance studies. Factors that influence dietary zinc requirement include dietary and other substances that either facilitate or inhibit absorption and retention of zinc; and metabolic phenomena that influence retention or excretion of the element. These determinants must be considered in estimating the requirement and the recommended dietary allowance for this essential element. An approach that has been used to assess requirement is the measurement of dietary zinc retention by men fed diets providing sufficient energy to meet the needs of each individual and containing other nutrients in proportion to energy content. By measurement of chemical balance and subsequent analysis of the data by multiple regression, dietary factors influencing requirement have been identified and amounts of dietary zinc essential for needs calculated. Using this approach, 83% of the variance (P less than 0.0001) in requirement was accounted for by the dietary content of phosphorus and nitrogen when data from 157 twenty-eight to thirty day studies were analyzed. The equation, Intake = 1.466 + 0.23 (Zn balance) + 5.19 (P intake) + 0.40 (N intake) - 0.30 (P intake - 1.389) (N intake - 14.646), was used to estimate zinc requirement of persons who participated in the most recent USDA Food Consumption Survey. For all age groups, ages 9 to greater than 75y, males and females, the mean (range) difference between the estimated intakes and calculated requirements were: males 1.5% (-6.5 to + 8.1), females 11.3% (- 1.1 to + 20.3). Thus, mean intakes were generally within the 95% confidence limits of the estimated requirements. Of some interest was the finding that the mean intake of none of the groups was equivalent to the Recommended Dietary Allowance. The mean intake of males ranged from 9.32 mg in men 75y and older to 13.53 mg in persons 15-18y. The mean intake of the females ranged from 7.04 mg in women 75y and older to 9.22 mg in persons 12-14 y.