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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
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Using PhyloSuite for molecular phylogeny and tree-based analyses.

Chuan-Yu Xiang1, Fangluan Gao2, Ivan Jakovlić1

  • 1State Key Laboratory of Grassland Agro-Ecosystems, and College of Ecology Lanzhou University Lanzhou China.

Imeta
|June 13, 2024
PubMed
Summary
This summary is machine-generated.

PhyloSuite simplifies complex multilocus phylogenetic analysis for researchers. This user-friendly software integrates essential tools, making phylogenomic reconstruction more accessible and efficient, especially for organelle-based studies.

Keywords:
annotationconcatenationiTOLlocimultiple‐sequence alignmentpartitioningtrimming

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Area of Science:

  • Genomics
  • Bioinformatics
  • Evolutionary Biology

Background:

  • Multilocus phylogenetic analysis is crucial in the genomics era.
  • Navigating numerous software programs for phylogenomic reconstruction is challenging for less experienced researchers.
  • PhyloSuite aims to simplify this process by integrating multiple software tools.

Purpose of the Study:

  • To provide a comprehensive protocol for conducting multilocus phylogenetic analyses using PhyloSuite.
  • To introduce PhyloSuite v1.2.3, highlighting bug fixes, optimizations, and new tree-based analysis functions.
  • To assist researchers, particularly those focusing on organelle-based phylogenies, in quick-starting their analyses.

Main Methods:

  • Utilizing PhyloSuite's graphical user interface (GUI) for an integrated phylogenetic pipeline.
  • Implementing step-by-step guidance covering background, rationale, and operational procedures for each analysis step.
  • Employing new functions in PhyloSuite v1.2.3 for advanced tree-based analyses.

Main Results:

  • PhyloSuite v1.2.3 offers a streamlined workflow for multilocus phylogenetic reconstruction.
  • New functionalities include signal-to-noise calculation, saturation analysis, and spurious species identification.
  • The protocol facilitates efficient execution of phylogenetic and tree-based analyses.

Conclusions:

  • PhyloSuite significantly lowers the barrier to entry for complex multilocus phylogenetic analyses.
  • The software and accompanying protocol empower researchers to conduct advanced phylogenomic studies more effectively.
  • This resource is particularly valuable for organelle genomics and researchers new to phylogenetics.