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    Annular-illumination quantitative phase imaging (AIKK) offers non-iterative phase measurement with enhanced resolution. A novel complementary illumination method records full object spectra in a single shot, improving phase measurement accuracy.

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    Area of Science:

    • Optics and Photonics
    • Image Processing
    • Metrology

    Background:

    • Quantitative phase imaging (QPI) is crucial for label-free biological sample analysis.
    • Traditional QPI methods often face limitations in resolution enhancement and spectral recording efficiency.
    • Annular-illumination quantitative phase imaging based on Kramers-Kronig relations (AIKK) offers a non-iterative approach.

    Purpose of the Study:

    • To develop a single-shot method for complete object spectrum recording in AIKK.
    • To enhance spatiotemporal resolution in quantitative phase measurements.
    • To overcome spectrum loss issues caused by spectral conjugation in multi-exposure methods.

    Main Methods:

    • Proposed a colorful complementary illumination method for simultaneous recording of spectral segments.
    • Utilized non-symmetrical illumination angles to ensure spectral complementarity across three channels.
    • Applied numerical filtering during reconstruction to correct for overlapped spectra.

    Main Results:

    • Successfully recorded full-angle information spectra across three wavelengths in a single exposure.
    • Demonstrated the ability to avoid spectrum loss due to spectral conjugation.
    • Achieved high spatiotemporal resolution quantitative phase measurements through simulations and experiments.

    Conclusions:

    • The proposed colorful complementary illumination method significantly improves spectral recording efficiency for AIKK.
    • This technique enables high-resolution, single-shot quantitative phase measurements.
    • The method provides a robust solution for overcoming spectral limitations in phase imaging.