Olive tree leaves as a great source of phenolic compounds: Comprehensive profiling of NaDES extracts

Waste from the olive industry is a noticeable source of antioxidant compounds that can be extracted and reused to produce raw materials related to the chemical, cosmetic, food and pharmaceutical sectors. This work studies the phenolic composition of olive leaf samples using liquid chromatography with ultraviolet detection coupled to mass spectrometry (LC-UV-MS). Olive leaf waste samples have been crushed, homogenized, and subjected to a solid-liquid extraction treatment with mechanical shaking at 80 °C for 2 h using Natural Deep Eutectic Solvents (NaDES). The phenolic compound identification in the resulting extracts has been carried out by high-resolution mass spectrometry (HRMS) using data-dependent acquisition mode using an Orbitrap HRMS instrument. >60 different phenolic compounds have been annotated tentatively, of which about 20 have been confirmed from the corresponding standards. Some of the most noticeable compounds are oleuropein and its aglycone and glucoside form, luteolin-7-O-glucoside, 3-hydroxytyrosol, and verbascoside.