Related Experiment Video
Updated: Jun 23, 2025
10:59
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
22.4K
The Blastulation Rate Is Negatively Associated With Euploid Rate.
Ido Feferkorn1, Jason Raina2, Eva Suarthana2
1Division of Reproductive Endocrinology and Infertility, McGill University Health Care Centre, Montréal, QC; Department of Obstetrics and Gynecology, Lis Maternity Hospital, Fertility Division, Tel-Aviv Medical Center affiliated to the Sackler Faculty of Medicine, Tel-Aviv, Israel.
Journal of Obstetrics and Gynaecology Canada : JOGC = Journal D'Obstetrique Et Gynecologie Du Canada : JOGC
|June 15, 2024
Summary
Female age and embryo development rates, including blastulation and the presence of one pronucleus (1PN) zygotes, negatively impact the euploid ratio in preimplantation genetic testing. Further research into embryo development mechanisms is needed.
Area of Science:
- Reproductive biology
- Embryology
- Genetics
Background:
- Preimplantation genetic testing (PGT) is crucial for assessing embryo ploidy.
- Understanding factors influencing blastocyst development and ploidy is essential for improving IVF outcomes.
- The relationship between early developmental markers and aneuploidy requires further elucidation.
Purpose of the Study:
- To investigate the association between blastulation rate, the presence of one pronucleus (1PN) zygotes, and the ploidy status of blastocysts.
- To identify key factors influencing the euploid ratio in human embryos.
Main Methods:
- Cross-sectional study utilizing existing databases from two Canadian university fertility centers.
- Analysis of 345 cycles from 235 couples undergoing next-generation sequencing PGT for aneuploidy detection.
- Biopsy of 1456 blastocysts for genetic analysis.
Main Results:
- Multivariate analysis revealed a negative association between female age and the number of 1PN/2PN embryos with the euploid ratio.
- Blastulation rate showed a surprising negative association with the euploid ratio when cycles with delayed blastulation were excluded.
- These findings suggest complex interactions between developmental timing and ploidy.
Conclusions:
- Early embryonic development stages, including blastulation and pronuclear status, are linked to blastocyst ploidy.
- Further investigation into the mechanisms of embryo development and cell cycle regulation is warranted.
- These insights may inform strategies for embryo selection in assisted reproductive technologies.
