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Saccharomyces cerevisiae structural cell wall mannoprotein.

J Frevert, C E Ballou

    Biochemistry
    |January 29, 1985
    PubMed
    Summary
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    Researchers isolated a novel mannoprotein from Saccharomyces cerevisiae cell walls. This protein

    Area of Science:

    • Microbiology
    • Biochemistry
    • Molecular Biology

    Background:

    • Saccharomyces cerevisiae cell wall mannoproteins play crucial roles in cellular structure and interactions.
    • The mnn9 mutant is known to have defects in mannoprotein synthesis and cell wall integrity.

    Purpose of the Study:

    • To isolate and characterize a novel mannoprotein fraction from the Saccharomyces cerevisiae mnn9 mutant cell wall.
    • To investigate the biosynthesis and translocation of this mannoprotein.

    Main Methods:

    • Solubilization of cell walls using beta-glucanase digestion and sodium dodecyl sulfate extraction.
    • Analysis of molecular weight and composition (protein and carbohydrate content).
    • Enzymatic digestion (Endoglucosaminidase H) and incorporation of radiolabeled proline ([3H]proline) to study biosynthesis.

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    Main Results:

    • A novel mannoprotein fraction (average MW 180,000) was isolated from the mnn9 mutant cell wall.
    • The mannoprotein consists of 12% protein (rich in proline) and 88% carbohydrate (primarily mannose).
    • Tunicamycin treatment resulted in a lower molecular weight mannoprotein (140,000) in both mutant and wild-type cells, indicating glycosylation's role in size determination.

    Conclusions:

    • The study successfully isolated and characterized a unique mannoprotein from Saccharomyces cerevisiae.
    • Glycosylation significantly influences the molecular weight and cell wall integration of mannoproteins.
    • Further research is needed to fully elucidate the structure and function of this novel mannoprotein and its associated peptide component.