Analyzing Pooled Microarray Gene Expression Data to Uncover Common Pathways in Periodontitis and Oral Squamous Cell
Roja Lakshmi Karri1, Rudraraju Amrutha2, Shrinivas2
1Department of Oral and Maxillofacial Pathology, GSL Dental College, Rajahmundry, Andhra Pradesh, India.
Journal of Pharmacy & Bioallied Sciences
|June 17, 2024
Summary
This study reveals shared molecular mechanisms between periodontitis and oral squamous cell carcinoma (OSCC). Key genes like CD19 and VWF, and specific microRNAs, may offer new therapeutic targets for these common oral diseases.
Area of Science:
- Oral pathology
- Molecular biology
- Genomics
Background:
- Periodontitis and oral squamous cell carcinoma (OSCC) are common oral diseases with different causes but shared biological traits.
- Understanding their molecular connections can reveal new treatment strategies.
Purpose of the Study:
- To identify common molecular mechanisms and potential therapeutic targets between periodontitis and OSCC.
- To analyze gene expression data and construct interaction networks.
Main Methods:
- Utilized gene expression datasets (GEO: GSE30784 for OSCC, GSE10334 for periodontitis).
- Performed differential gene expression analysis using GEO2R.
- Constructed a protein-protein interaction (PPI) network via STRING and visualized with Cytoscape.
- Identified key microRNAs (miRNAs) using miRNet.
Main Results:
- Identified common differentially expressed genes (DEGs) between periodontitis and OSCC.
- Cluster of Differentiation 19 (CD19), Von Willebrand Factor (VWF), and Complement C3 (C3) emerged as top hub genes.
- Enriched pathways included B-cell receptor signaling and complement/coagulation cascades.
- Key miRNAs (hsa-mir-26a-5p, hsa-mir-129-2-3p, hsa-mir-27a-3p) were associated with these pathways.
Conclusions:
- Periodontitis and OSCC share underlying molecular pathways.
- The identified hub genes (CD19, VWF, C3) and miRNAs represent potential biomarkers and therapeutic targets for both conditions.
