Multifunctional Laser-Induced Graphene-Based Microfluidic Chip for High-Performance Oocyte Cryopreservation with Low...

Area of Science:

• Biotechnology
• Materials Science
• Reproductive Biology

Background:

• Oocyte cryopreservation is crucial for assisted reproduction but faces challenges due to oocyte size and sensitivity.
• Current cryopreservation methods require further improvement for better cell survival rates.

Purpose of the Study:

• To develop an improved cryopreservation method for oocytes using a novel microfluidic chip.
• To investigate the efficacy of a 3D graphene-based microfluidic chip in enhancing oocyte cryopreservation outcomes.

Main Methods:

• A Y-shaped microfluidic chip was fabricated using laser-induced graphene (LIG) and fiber etching technologies.
• The chip's surface hydrophobicity was adjusted to control ice crystal formation and freezing time.
• A photothermal effect was utilized via low-power laser irradiation to rapidly increase chip temperature.

Main Results:

• The LIG/PDMS microfluidic chip effectively suppressed ice crystal size and delayed freezing.
• The chip demonstrated a significant photothermal effect, increasing temperature rapidly with laser application.
• Reduced cryoprotectant concentrations (7.5% EG, 7.5% DMSO) resulted in a high oocyte survival rate of 92.4%, surpassing the control group (85.8%).

Conclusions:

• The developed LIG/PDMS microfluidic chip offers a novel approach for high-performance oocyte cryopreservation.
• This technology can replace traditional carriers and reduce cryoprotectant requirements, improving efficiency and survival rates.