Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryptococcal Meningitis

Cryptococcal Meningitis

Cryptococcal meningitis is a life-threatening opportunistic infection predominantly associated with HIV/AIDS, accounting for over 100,000 deaths annually worldwide. However, it also affects individuals with other forms of immunosuppression, including those undergoing immunosuppressive therapy, organ transplant recipients, patients with innate immunodeficiencies, and individuals with hematological disorders. The infection is caused mainly by Cryptococcus neoformans and Cryptococcus gattii,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Effect of Prevaccination Analgesics on Influenza Vaccine Immunogenicity and Effectiveness.

The Journal of infectious diseases·2026

Same author

Is cellular senescence a biological feature of Long COVID? A transcriptomic analysis across comparative post-acute sequelae phenotypes.

The Journal of infectious diseases·2026

Same author

Host Genetic Regulation of NLRP3 Inflammasome Cytokines Reveals Immune and Vascular Pathways in HIV.

medRxiv : the preprint server for health sciences·2026

Same author

Correction: SARS-CoV-2 infection is associated with self-reported post-acute neuropsychological symptoms within six months of follow-up.

PloS one·2026

Same author

Targeting the H/KRAS α4-β6-α5 Allosteric Lobe with Macrocyclic Peptides.

ACS medicinal chemistry letters·2026

Same author

Influenza-like illness in cigarette smokers and electronic-cigarette users: a secondary analysis from the PAIVED study.

Frontiers in public health·2026

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

A Seminested PCR Method for the Diagnosis of Invasive Fungal Infections in Combat Injured.

Graham C Ellis¹, Faraz Shaikh^2,3, M Leigh Carson^2,3

¹Walter Reed National Military Medical Center, Bethesda, Maryland, USA.

Open Forum Infectious Diseases

|June 18, 2024

Summary

New seminested assays show promise for diagnosing invasive fungal infections (IFIs) in combat-injured patients. These molecular methods offer a valuable alternative to traditional diagnostics, particularly for soft-tissue IFIs with angioinvasion.

Keywords:

PCR-based assays combat invasive fungal wound infection mucormycosis trauma

More Related Videos

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

Related Experiment Videos

Last Updated: Jun 7, 2026

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

Area of Science:

Medical Mycology
Molecular Diagnostics
Infectious Diseases

Background:

Invasive fungal infections (IFIs) cause significant morbidity in combat-injured individuals.
Traditional diagnostic methods like cultures and histopathology for IFIs have limitations.
Previous research established the efficacy of a panfungal polymerase chain reaction (PCR) assay for angioinvasive IFIs.

Purpose of the Study:

To evaluate three less resource-intensive seminested PCR assays.
To target clinically relevant fungi: order Mucorales, and genera *Aspergillus* and *Fusarium*.
To assess the utility of these assays in formalin-fixed paraffin-embedded tissue samples from combat-injured patients.

Main Methods:

Utilized formalin-fixed paraffin-embedded tissue specimens from a multicenter trauma IFI cohort (2009-2014).
Compared seminested assays (targeting Mucorales, *Aspergillus*, *Fusarium*) with a panfungal assay and histopathology.
Included cases of US military personnel with histopathologic IFI evidence and controls with no laboratory IFI evidence.

Main Results:

Observed substantial agreement between seminested and panfungal assays, particularly for Mucorales.
Found moderate agreement at the genus level for *Aspergillus* and *Fusarium*.
Seminested assays demonstrated 67.4% sensitivity and 96.6% specificity compared to histopathology, with sensitivity reaching 91.7% for angioinvasive sites.

Conclusions:

The seminested approach is useful for diagnosing soft-tissue IFIs in formalin-fixed paraffin-embedded tissue.
Findings support the utility of seminested molecular diagnostics in a combat-injured population.
This approach is particularly effective for IFIs with angioinvasion.