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RIP5 Interacts with REL1 and Negatively Regulates Drought Tolerance in Rice
Qiuxin Zhang1, Dan He1, Jingjing Zhang1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
Cells
|June 19, 2024
Summary
Rice plants gain drought resistance when ROLLED AND ERECT LEAF1 (REL1) inhibits RIP5. This mechanism enhances reactive oxygen species (ROS) clearance by increasing ascorbic acid (AsA) levels, crucial for stable rice yields.
Area of Science:
- Plant Biology
- Molecular Biology
- Agricultural Science
Background:
- Improving rice drought resistance is vital for food security.
- ROLLED AND ERECT LEAF1 (REL1) previously showed a role in drought tolerance by managing reactive oxygen species (ROS) and abscisic acid (ABA) response.
- The precise mechanism of REL1 in ROS scavenging during drought remained unclear.
Purpose of the Study:
- To elucidate the mechanism by which REL1 enhances drought tolerance through ROS removal.
- To identify and characterize proteins interacting with REL1 in the context of drought stress.
- To investigate the role of RIP5 in rice drought response.
Main Methods:
- Protein-protein interaction assays to identify REL1 interacting partners.
- Analysis of RIP5 expression under drought conditions in rice (ZH11).
- Phenotypic analysis of rice mutants (rip5-ko) and overexpression lines under drought stress.
- Measurement of ascorbic acid (AsA) content and ROS levels.
- RNA sequencing to analyze gene expression changes in rip5-ko mutants.
Main Results:
- REL1 interacting protein 5 (RIP5) was identified as a direct binding partner of REL1 in chloroplasts.
- RIP5 expression is upregulated under drought stress, and rip5-ko mutants exhibit enhanced drought tolerance, while RIP5 overexpression lines are more susceptible.
- RIP5 negatively regulates drought tolerance by reducing ascorbic acid (AsA) content, impairing ROS clearance.
- RNA sequencing revealed that RIP5 knockout significantly impacts genes involved in ascorbate and aldarate metabolism.
- REL1 appears to enhance drought tolerance by inhibiting the function of RIP5.
Conclusions:
- REL1 confers drought tolerance by inhibiting RIP5, which in turn increases ascorbic acid levels and improves ROS scavenging.
- This study reveals a novel regulatory pathway involving REL1 and RIP5 in rice drought stress response.
- Understanding this mechanism provides a basis for developing strategies to enhance rice drought resistance.
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