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Gas Chromatography–Mass Spectrometry (GC–MS)01:14

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Recent advances in gas phase unfolding: Instrumentation and applications.

Rowan Matney1, Varun V Gadkari1

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PubMed
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Native mass spectrometry (MS) and ion mobility-mass spectrometry (IM-MS) enable gas-phase analysis of biomolecules. Collision-induced unfolding (CIU) is a key technique for studying structural stability changes in response to various stimuli.

Keywords:
collision induced unfoldingcyclic ion mobilitygas phase unfoldingnative ion mobility mass spectrometrynative liquid chromatographynative mass spectrometryproteinsstability

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Structural Biology

Background:

  • Native mass spectrometry (MS) and ion mobility-mass spectrometry (IM-MS) are increasingly used for gas-phase analysis of complex biomolecules.
  • Collision-induced unfolding (CIU) is a powerful technique that leverages MS capabilities to probe the structural stability of biomolecules.
  • Advances in IM-MS instrumentation and novel research methodologies have facilitated the growth of CIU applications.

Purpose of the Study:

  • To highlight recent advancements in collision-induced unfolding (CIU) techniques.
  • To discuss the role of native mass spectrometry (MS) and ion mobility-mass spectrometry (IM-MS) in gas-phase structural biology.
  • To provide a forward-looking perspective on the future of gas-phase unfolding studies.

Main Methods:

  • Utilizing native mass spectrometry (MS) for gas-phase separation and detection of biomolecules.
  • Employing ion mobility-mass spectrometry (IM-MS) to enhance the resolution and analytical capabilities for structural studies.
  • Applying collision-induced unfolding (CIU) to assess changes in biomolecular structural stability.

Main Results:

  • Demonstrated the capability of CIU to detect subtle shifts in biomolecular structural stability.
  • Showcased how MS and IM-MS enable sensitive and selective measurements of gas-phase biomolecular structures.
  • Highlighted the impact of instrumentation advances and innovative methods on the field of gas-phase unfolding.

Conclusions:

  • Collision-induced unfolding (CIU), powered by MS and IM-MS, is a rapidly advancing field for studying biomolecular structure and stability.
  • Future developments in instrumentation and methodology promise to further expand the scope and impact of gas-phase unfolding techniques.
  • This perspective underscores the growing importance of gas-phase unfolding in understanding biological molecules.