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Related Experiment Videos

Replicating instabilities in yeast: occurrence in different mutational systems.

A Nasim, E R Stephen, J A Erratt

    Molecular & General Genetics : MGG
    |January 1, 1985
    PubMed
    Summary

    Yeast cells treated with nitrosoguanidine showed unstable genetic elements. These replicating instabilities were observed across multiple locations in the Schizosaccharomyces pombe genome and also in Saccharomyces cerevisiae.

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    Area of Science:

    • Genetics
    • Molecular Biology
    • Yeast Research

    Background:

    • Mutagenesis studies are crucial for understanding genome stability.
    • Phenotypic variation in yeast can indicate underlying genetic instability.
    • Schizosaccharomyces pombe and Saccharomyces cerevisiae are model organisms for genetic research.

    Purpose of the Study:

    • To investigate the occurrence and nature of genetic instabilities in yeast.
    • To determine if these instabilities are widespread across the genome.
    • To confirm the phenomenon in different yeast species.

    Main Methods:

    • Yeast cells (Schizosaccharomyces pombe and Saccharomyces cerevisiae) were treated with nitrosoguanidine.
    • Primary mosaic colonies with prototrophic/auxotrophic phenotypes were isolated.

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  • Progeny of primary mosaics were replated to identify secondary mosaics.
  • The ade-1 gene in Saccharomyces cerevisiae was used as a marker for pigmentation changes.

    • Main Results:

    • Nitrosoguanidine mutagenesis induced primary mosaic yeast colonies.
    • Replication of these primary mosaics yielded numerous secondary mosaics.
    • Genetic instabilities were found to occur at multiple loci within the Schizosaccharomyces pombe genome.
    • The phenomenon of replicating instabilities was also observed in Saccharomyces cerevisiae using the ade-1 gene.

    Conclusions:

    • Replicating genetic instabilities are a significant feature in the Schizosaccharomyces pombe genome.
    • This phenomenon is not exclusive to Schizosaccharomyces pombe and occurs in Saccharomyces cerevisiae as well.
    • The study highlights the dynamic nature of yeast genomes and the potential for widespread genetic instability.