Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replicating instabilities in yeast: occurrence in different mutational systems.

A Nasim, E R Stephen, J A Erratt

    Molecular & General Genetics : MGG
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inclusion of Veterinary Services in national emergency management plans.

    Revue scientifique et technique (International Office of Epizootics)·2020
    Same author

    Cementing the vena cava.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2015
    Same author

    Digital ischaemia in the digital age.

    Journal of surgical case reports·2014
    Same author

    Procedural risk following carotid endarterectomy in the hyperacute period after onset of symptoms.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2013
    Same author

    Clinical presentation and outcome of dengue viral infection in live-related renal transplant recipients in Karachi, Pakistan.

    Transplant infectious disease : an official journal of the Transplantation Society·2013
    Same author

    Closing the loop: a 21-year audit of strategies for preventing stroke and death following carotid endarterectomy.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2013
    Same journal

    A multigene family in Calothrix sp. PCC 7601 encodes phycocyanin, the major component of the cyanobacterial light-harvesting antenna

    Molecular & general genetics : MGG·2017
    Same journal

    Mutants of Schizosaccharomyces pombe which sporulate in the haploid state.

    Molecular & general genetics : MGG·2015
    Same journal

    The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats.

    Molecular & general genetics : MGG·2014
    Same journal

    Multiple mutant analysis of recombination in yeast.

    Molecular & general genetics : MGG·2012
    Same journal

    Intergenic suppression of spoO phenotypes by the Bacillus subtilis mutation rvtA.

    Molecular & general genetics : MGG·2010
    Same journal

    Expression of the seqA gene is negatively modulated by the HU protein in Escherichia coli.

    Molecular & general genetics : MGG·2001
    See all related articles

    Yeast cells treated with nitrosoguanidine showed unstable genetic elements. These replicating instabilities were observed across multiple locations in the Schizosaccharomyces pombe genome and also in Saccharomyces cerevisiae.

    Area of Science:

    • Genetics
    • Molecular Biology
    • Yeast Research

    Background:

    • Mutagenesis studies are crucial for understanding genome stability.
    • Phenotypic variation in yeast can indicate underlying genetic instability.
    • Schizosaccharomyces pombe and Saccharomyces cerevisiae are model organisms for genetic research.

    Purpose of the Study:

    • To investigate the occurrence and nature of genetic instabilities in yeast.
    • To determine if these instabilities are widespread across the genome.
    • To confirm the phenomenon in different yeast species.

    Main Methods:

    • Yeast cells (Schizosaccharomyces pombe and Saccharomyces cerevisiae) were treated with nitrosoguanidine.
    • Primary mosaic colonies with prototrophic/auxotrophic phenotypes were isolated.

    Related Experiment Videos

  • Progeny of primary mosaics were replated to identify secondary mosaics.
  • The ade-1 gene in Saccharomyces cerevisiae was used as a marker for pigmentation changes.

    • Main Results:

    • Nitrosoguanidine mutagenesis induced primary mosaic yeast colonies.
    • Replication of these primary mosaics yielded numerous secondary mosaics.
    • Genetic instabilities were found to occur at multiple loci within the Schizosaccharomyces pombe genome.
    • The phenomenon of replicating instabilities was also observed in Saccharomyces cerevisiae using the ade-1 gene.

    Conclusions:

    • Replicating genetic instabilities are a significant feature in the Schizosaccharomyces pombe genome.
    • This phenomenon is not exclusive to Schizosaccharomyces pombe and occurs in Saccharomyces cerevisiae as well.
    • The study highlights the dynamic nature of yeast genomes and the potential for widespread genetic instability.