Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An electrophoretic karyotype for yeast.

G F Carle, M V Olson

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Institutional Biobanking as shared public health infrastructure: a financially sustainable service center model.

    Frontiers in health services·2026
    Same author

    Balancing Sustainability and Specimen Protection.

    F1000Research·2026
    Same author

    Mdm2 controls CREB-dependent transactivation and initiation of adipocyte differentiation.

    Cell death and differentiation·2012
    Same author

    Molecular effects of gallium on osteoclastic differentiation of mouse and human monocytes.

    Biochemical pharmacology·2011
    Same author

    Field-inversion gel electrophoresis.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Immune changes in post-menopausal osteoporosis: the Immunos study.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2009
    Same journal

    The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Superstatistics approach to turbulent circulation fluctuations.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    A molecular timescale for evolution of cobamide biosynthesis.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Granulosa cell glycogen fuels the avascular corpus luteum.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Researchers created an electrophoretic karyotype for Saccharomyces cerevisiae, mapping 15 chromosomes using orthogonal-field-alternation gel electrophoresis and DNA hybridization. This technique provides a comprehensive view of yeast chromosomal DNA.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Yeast Genomics

    Background:

    • Standard laboratory yeast strains possess complex chromosomal structures.
    • Accurate mapping of these chromosomes is crucial for genetic studies.

    Purpose of the Study:

    • To develop a high-resolution electrophoretic karyotype for Saccharomyces cerevisiae.
    • To correlate DNA banding patterns with genetically defined chromosomes.

    Main Methods:

    • Orthogonal-field-alternation gel electrophoresis (OFAGE) was employed to separate chromosomal DNA molecules.
    • DNA-DNA hybridization with cloned gene probes was used for chromosome identification.
    • Analysis was performed on a standard yeast strain and compared with strains exhibiting polymorphisms.

    Related Experiment Videos

    Main Results:

    • Twelve well-resolved bands representing 15 chromosomes (I-XI, XIII-XVI) were identified.
    • Nine bands were singlets, and three were comigrating doublets, resolvable in polymorphic strains.
    • Chromosome XII (rRNA genes) did not consistently enter the gel.
    • A previously unmapped fragment (F8) was assigned to chromosome XIII.

    Conclusions:

    • The study established a comprehensive electrophoretic karyotype for yeast.
    • OFAGE combined with hybridization provides a powerful tool for yeast chromosome analysis.
    • This karyotype serves as a foundational map for further yeast genetic research.